Volume 8, Issue 2, December 2020, Page: 40-46
Copper Oxide Nanoparticles: Reactive Oxygen Species Generation and Biomedical Applications
Sadaf Sarfraz, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Akmal Javed, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Shahzad Sharif Mughal, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Muzammil Bashir, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Abdul Rehman, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Sajida Parveen, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Anam Khushi, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Muhammad Kamran Khan, Department of Botany, Government College University, Faisalabad, Punjab, Pakistan
Received: Jun. 22, 2020;       Accepted: Nov. 3, 2020;       Published: Nov. 19, 2020
DOI: 10.11648/j.ijctc.20200802.12      View  7      Downloads  3
Copper oxide is a p-type semiconductor which has many applications in a different field. Copper oxide has excellent applications as an antioxidant, antibacterial, and antitumor or anticancer. Copper oxide nanoparticle combines with the cell membrane and enters into a cell; generate reactive oxygen specie (ROS), which causes oxidative stress in the cell. Oxidative stress leads to metastasis, cancer proliferation, apoptosis, DNA damage, cytotoxicity, and unregulated cell signaling. Hydroxyl free radical generated by Nanoparticles, combined with DNA and yield 8-hydroxyl-2-deoxyguanosine (8-OHdG), resultantly DNA is damaged. CuO nanoparticle shows antibacterial activity on different bacterial strains such as staphylococcus aureus, bacillus circulens BP2, Escherichia coli, and P. aeruginosa. Recently, CuO nanoparticles have applications in the detection of Cholesterol, lactate biosensor, DNA sequencing of microbe, and anti-HIV drug analysis. There is specialized CuO nanoparticle such as Glucose sensor, Hydrogen peroxide sensor, Immunosensor, Dopamine sensor for the detection of the different biomolecule. ROS generated by CuO nanoparticle causes toxicity, which leads to cell death. There is a fascinating area of research against tumors by nanoparticle use because of its antitumor nature. Metal nanoparticle exhibits anticancer activity due to physicochemical properties as antioxidant action or use of external stimuli. Free radical which are produced by the metal nanoparticle, kill cancer cells.
Copper Oxide Nanoparticle, Reactive Oxygen Specie (ROS), Sensors, Cancer Therapy, Biomedical Applications, Cytotoxicity and Toxicity
To cite this article
Sadaf Sarfraz, Akmal Javed, Shahzad Sharif Mughal, Muzammil Bashir, Abdul Rehman, Sajida Parveen, Anam Khushi, Muhammad Kamran Khan, Copper Oxide Nanoparticles: Reactive Oxygen Species Generation and Biomedical Applications, International Journal of Computational and Theoretical Chemistry. Vol. 8, No. 2, 2020, pp. 40-46. doi: 10.11648/j.ijctc.20200802.12
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sankar, R., et al., Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Materials Science and Engineering: C, 2014. 44: p. 234-239.
Ren, G., et al., Characterisation of copper oxide nanoparticles for antimicrobial applications. International journal of antimicrobial agents, 2009. 33 (6): p. 587-590.
Tranquada, J., et al., Evidence for stripe correlations of spins and holes in copper oxide superconductors. nature, 1995. 375 (6532): p. 561.
Chang, Y.-N., et al., The toxic effects and mechanisms of CuO and ZnO nanoparticles. Materials, 2012. 5 (12): p. 2850-2871.
Perreault, F., et al., Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures. Science of the Total Environment, 2012. 441: p. 117-124.
Cioffi, N., et al., Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties. Chemistry of Materials, 2005. 17 (21): p. 5255-5262.
Caputo, F., M. De Nicola, and L. Ghibelli, Pharmacological potential of bioactive engineered nanomaterials. Biochemical pharmacology, 2014. 92 (1): p. 112-130.
Danhier, F., O. Feron, and V. Préat, To exploit the tumor microenvironment: passive and active tumor targeting of nanocarriers for anti-cancer drug delivery. Journal of controlled release, 2010. 148 (2): p. 135-146.
Baek, Y.-W. and Y.-J. An, Microbial toxicity of metal oxide nanoparticles (CuO, NiO, ZnO, and Sb2O3) to Escherichia coli, Bacillus subtilis, and Streptococcus aureus. Science of the total environment, 2011. 409 (8): p. 1603-1608.
Isani, G., et al., Comparative toxicity of CuO nanoparticles and CuSO4 in rainbow trout. Ecotoxicology and environmental safety, 2013. 97: p. 40-46.
Ruiz, P., et al., Short-term effects on antioxidant enzymes and long-term genotoxic and carcinogenic potential of CuO nanoparticles compared to bulk CuO and ionic copper in mussels Mytilus galloprovincialis. Marine environmental research, 2015. 111: p. 107-120.
Touyz, R. M., Molecular and cellular mechanisms in vascular injury in hypertension: role of angiotensin II–editorial review. Current opinion in nephrology and hypertension, 2005. 14 (2): p. 125-131.
Finkel, T., Signal transduction by mitochondrial oxidants. Journal of Biological Chemistry, 2012. 287 (7): p. 4434-4440.
Johnson, F. and C. Giulivi, Superoxide dismutases and their impact upon human health. Molecular aspects of medicine, 2005. 26 (4-5): p. 340-352.
Ray, P. D., B.-W. Huang, and Y. Tsuji, Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular signalling, 2012. 24 (5): p. 981-990.
Guzik, T. J. and D. G. Harrison, Vascular NADPH oxidases as drug targets for novel antioxidant strategies. Drug discovery today, 2006. 11 (11-12): p. 524-533.
Coso, S., et al., NADPH oxidases as regulators of tumor angiogenesis: current and emerging concepts. Antioxidants & redox signaling, 2012. 16 (11): p. 1229-1247.
Bedard, K. and K.-H. Krause, The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiological reviews, 2007. 87 (1): p. 245-313.
Storz, P., Forkhead homeobox type O transcription factors in the responses to oxidative stress. Antioxidants & redox signaling, 2011. 14 (4): p. 593-605.
Halliwell, B. and J. M. Gutteridge, Free radicals in biology and medicine. 2015: Oxford University Press, USA.
Mignolet-Spruyt, L., et al., Spreading the news: subcellular and organellar reactive oxygen species production and signalling. Journal of experimental botany, 2016. 67 (13): p. 3831-3844.
Aust, S., et al., Free radicals in toxicology. Toxicology and applied pharmacology, 1993. 120 (2): p. 168-178.
Thannickal, V. J. and B. L. Fanburg, Reactive oxygen species in cell signaling. American Journal of Physiology-Lung Cellular and Molecular Physiology, 2000. 279 (6): p. L1005-L1028.
Xia, T., et al., Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano letters, 2006. 6 (8): p. 1794-1807.
Risom, L., P. Møller, and S. Loft, Oxidative stress-induced DNA damage by particulate air pollution. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2005. 592 (1-2): p. 119-137.
Rahman, K., Studies on free radicals, antioxidants, and co-factors. Clinical interventions in aging, 2007. 2 (2): p. 219.
Fenoglio, I., et al., The oxidation of glutathione by cobalt/tungsten carbide contributes to hard metal-induced oxidative stress. Free radical research, 2008. 42 (8): p. 437-745.
Zhu, X., et al., Biosensing approaches for rapid genotoxicity and cytotoxicity assays upon nanomaterial exposure. Small, 2013. 9 (9-10): p. 1821-1830.
Nel, A., et al., Toxic potential of materials at the nanolevel. science, 2006. 311 (5761): p. 622-627.
Xia, T., et al., Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS nano, 2008. 2 (10): p. 2121-2134.
Valavanidis, A., T. Vlachogianni, and C. Fiotakis, 8-hydroxy-2′-deoxyguanosine (8-OHdG): a critical biomarker of oxidative stress and carcinogenesis. Journal of environmental science and health Part C, 2009. 27 (2): p. 120-139.
Shukla, R. K., et al., ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicology in vitro, 2011. 25 (1): p. 231-241.
Turski, M. L. and D. J. Thiele, New roles for copper metabolism in cell proliferation, signaling, and disease. Journal of Biological Chemistry, 2009. 284 (2): p. 717-721.
Li, Y., et al., Chronic Al2O3-nanoparticle exposure causes neurotoxic effects on locomotion behaviors by inducing severe ROS production and disruption of ROS defense mechanisms in nematode Caenorhabditis elegans. Journal of hazardous materials, 2012. 219: p. 221-230.
Vallyathan, V. and X. Shi, The role of oxygen free radicals in occupational and environmental lung diseases. Environmental Health Perspectives, 1997. 105 (suppl 1): p. 165-177.
Das, D., et al., Synthesis and evaluation of antioxidant and antibacterial behavior of CuO nanoparticles. Colloids and Surfaces B: Biointerfaces, 2013. 101: p. 430-433.
Borkow, G. and J. Gabbay, Copper as a biocidal tool. Current medicinal chemistry, 2005. 12 (18): p. 2163-2175.
Gant, V. A., et al., Three novel highly charged copper-based biocides: safety and efficacy against healthcare-associated organisms. Journal of Antimicrobial Chemotherapy, 2007. 60 (2): p. 294-299.
Kawanishi, S., Y. Hiraku, and S. Oikawa, Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging. Mutation Research/Reviews in Mutation Research, 2001. 488 (1): p. 65-76.
Borkow, G., et al., Deactivation of human immunodeficiency virus type 1 in medium by copper oxide-containing filters. Antimicrobial agents and chemotherapy, 2008. 52 (2): p. 518-525.
Borkow, G., et al., A novel anti-influenza copper oxide containing respiratory face mask. PLoS One, 2010. 5 (6): p. e11295.
AshaRani, P., et al., Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS nano, 2008. 3 (2): p. 279-290.
Singh, J., et al., Bienzyme-functionalized monodispersed biocompatible cuprous oxide/chitosan nanocomposite platform for biomedical application. The Journal of Physical Chemistry B, 2012. 117 (1): p. 141-152.
Sharma, V. and S. M. Mobin, Cytocompatible peroxidase mimic CuO: graphene nanosphere composite as colorimetric dual sensor for hydrogen peroxide and cholesterol with its logic gate implementation. Sensors and Actuators B: Chemical, 2017. 240: p. 338-348.
Uzunoglu, A. and L. A. Stanciu, Novel CeO2–CuO-decorated enzymatic lactate biosensors operating in low oxygen environments. Analytica chimica acta, 2016. 909: p. 121-128.
Chen, M., et al., An ultrasensitive electrochemical DNA biosensor based on a copper oxide nanowires/single-walled carbon nanotubes nanocomposite. Applied Surface Science, 2016. 364: p. 703-709.
Shahrokhian, S., et al., Electrodeposition of Copper Oxide Nanoparticles on Precasted Carbon Nanoparticles Film for Electrochemical Investigation of anti-HIV Drug Nevirapine. Electroanalysis, 2015. 27 (8): p. 1989-1997.
Xu, D., et al., Design and fabrication of Ag-CuO nanoparticles on reduced graphene oxide for nonenzymatic detection of glucose. Sensors and Actuators B: Chemical, 2018. 265: p. 435-442.
Yazid, S. N. A. M., I. M. Isa, and N. Hashim, Novel alkaline-reduced cuprous oxide/graphene nanocomposites for non-enzymatic amperometric glucose sensor application. Materials Science and Engineering: C, 2016. 68: p. 465-473.
Hasan, A., et al., Recent advances in application of biosensors in tissue engineering. BioMed research international, 2014. 2014.
Yuan, R., et al., Stable controlled growth of 3D CuO/Cu nanoflowers by surfactant-free method for non-enzymatic hydrogen peroxide detection. Journal of materials science & technology, 2018. 34 (9): p. 1692-1698.
Li, F., et al., Ultrasensitive amperometric immunosensor for PSA detection based on Cu2O@ CeO2-Au nanocomposites as integrated triple signal amplification strategy. Biosensors and Bioelectronics, 2017. 87: p. 630-637.
Wang, Y., et al., Simple synthesis of silver nanoparticles functionalized cuprous oxide nanowires nanocomposites and its application in electrochemical immunosensor. Sensors and Actuators B: Chemical, 2016. 236: p. 241-248.
Li, F., et al., Facile synthesis of MoS2@ Cu2O-Pt nanohybrid as enzyme-mimetic label for the detection of the Hepatitis B surface antigen. Biosensors and Bioelectronics, 2018. 100: p. 512-518.
Feng, T., et al., A porous CuO nanowire-based signal amplification immunosensor for the detection of carcinoembryonic antigens. Rsc Advances, 2016. 6 (21): p. 16982-16987.
Wang, Y., et al., Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein. RSC Advances, 2015. 5 (70): p. 56583-56589.
Sun, G., et al., CuO-induced signal amplification strategy for multiplexed photoelectrochemical immunosensing using CdS sensitized ZnO nanotubes arrays as photoactive material and AuPd alloy nanoparticles as electron sink. Biosensors and Bioelectronics, 2015. 66: p. 565-571.
Ghodsi, J., et al., Determination of dopamine in the presence of uric acid and folic acid by carbon paste electrode modified with CuO nanoparticles/hemoglobin and multi-walled carbon nanotube. Journal of The Electrochemical Society, 2015. 162 (4): p. B69-B74.
Zhang, F., et al., One-pot solvothermal synthesis of a Cu 2 O/graphene nanocomposite and its application in an electrochemical sensor for dopamine. Microchimica Acta, 2011. 173 (1-2): p. 103-109.
Felix, S., et al., Electrocatalytic oxidation of carbohydrates and dopamine in alkaline and neutral medium using CuO nanoplatelets. Journal of Electroanalytical Chemistry, 2015. 739: p. 1-9.
Wu, L.-N., et al., Dopamine sensor based on a hybrid material composed of cuprous oxide hollow microspheres and carbon black. Microchimica Acta, 2015. 182 (7-8): p. 1361-1369.
Liu, B., et al., Electrochemical preparation of nickel and copper oxides-decorated graphene composite for simultaneous determination of dopamine, acetaminophen and tryptophan. Talanta, 2016. 146: p. 114-121.
Yang, S., et al., Nano-sized copper oxide/multi-wall carbon nanotube/Nafion modified electrode for sensitive detection of dopamine. Journal of Electroanalytical Chemistry, 2013. 703: p. 45-51.
Wang, Y., et al., Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells. International journal of nanomedicine, 2012. 7: p. 2641.
Sankar, R., et al., Inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in Wistar Albino rats. Journal of Materials Science: Materials in Medicine, 2015. 26 (7): p. 214.
Song, H., et al., Serum adsorption, cellular internalization and consequent impact of cuprous oxide nanoparticles on uveal melanoma cells: implications for cancer therapy. Nanomedicine, 2015. 10 (24): p. 3547-3562.
Jeronsia, J. E., et al., In vitro antibacterial and anticancer activity of copper oxide nanostructures in human breast cancer Michigan Cancer Foundation-7 cells. Journal of Medical Sciences, 2016. 36 (4): p. 145.
Siddiqui, M. A., et al., Copper oxide nanoparticles induced mitochondria mediated apoptosis in human hepatocarcinoma cells. PloS one, 2013. 8 (8): p. e69534.
Joshi, A., et al., Uptake and toxicity of copper oxide nanoparticles in C6 glioma cells. Neurochemical research, 2016. 41 (11): p. 3004-3019.
Yang, Q., et al., Cuprous oxide nanoparticles trigger ER stress-induced apoptosis by regulating copper trafficking and overcoming resistance to sunitinib therapy in renal cancer. Biomaterials, 2017. 146: p. 72-85.
Wang, Y., et al., Cuprous oxide nanoparticles inhibit the growth and metastasis of melanoma by targeting mitochondria. Cell death & disease, 2013. 4 (8): p. e783.
Zhang, Q., et al., CuO nanostructures: synthesis, characterization, growth mechanisms, fundamental properties, and applications. Progress in Materials Science, 2014. 60: p. 208-337.
Karlsson, H. L., et al., Cell membrane damage and protein interaction induced by copper containing nanoparticles—Importance of the metal release process. Toxicology, 2013. 313 (1): p. 59-69.
Ahir, M., et al., Tailored-CuO-nanowire decorated with folic acid mediated coupling of the mitochondrial-ROS generation and miR425-PTEN axis in furnishing potent anti-cancer activity in human triple negative breast carcinoma cells. Biomaterials, 2016. 76: p. 115-132.
Chibber, S., S. A. Ansari, and R. Satar, New vision to CuO, ZnO, and TiO 2 nanoparticles: their outcome and effects. Journal of Nanoparticle Research, 2013. 15 (4): p. 1492.
Rodhe, Y., et al., Copper-based nanoparticles induce high toxicity in leukemic HL60 cells. Toxicology in Vitro, 2015. 29 (7): p. 1711-1719.
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